Productive assessment Essay Example | Topics and Well Written Essays - 500 words

Productive assessment - Essay Example As a result he was able to see the different features and benefits that each product had to offer. Consequently, he could deduce the marketing rationale of why a certain model was more or less than another and what features one could expect to find on the model in the lower, median, and high end price ranges. Dee Fink of the Oklahoma Instructional Development Program labels this approach â€Å"Doing† because Donald’s exposure to the products permits him to categorize them and create an appropriate hierarchy in his mind from which to delineate a value for each product that he can understand and remember. Dr. Charles Bonwell conducts Active Learning workshops where he helps school teachers learn this technique. According to Dr. Bonwell, the purpose of his workshops is to train instructors to move away from the classical approach of relying almost exclusively on the lecture as the primary learning tool to moving into an environment where the student participates directly i n all aspects of his/her learning experience.

Nutrition and Autism Assignment Example | Topics and Well Written Essays - 500 words - 1

Nutrition and Autism - Assignment Example Moreover, the elimination of lead-loaded petro has been identified as a deliberate campaign program that has been adopted to eliminate the adverse effects of the chemical substances. The article, therefore, links development of autism t exposure to chemical agents and thus its prevention lies squarely on elimination of exposure. Besides, the author identifies that children are more prone to these chemical agents. Exposure is mainly through maternal transfer to the baby during pregnancy or consistent early exposure to these chemicals. Whichever the case, there are over 200 chemicals that have been proven in laboratories to inhibit brain development. However, there is a critical impediment identified by the author as stumbling a block in avoidance of these chemicals. Firstly, the threshold to prove is too high and require study sample with the control group that is impossible due to ethical consideration. Besides, there are still gaps in chemical neurotoxicity tests leading to insufficient literature (Grandjean and Landrigan 2169). Despite the shortcomings, the article suggests removal of food items that contain these dangerous elements. I feel that the article is scientific and ought to offer direction to food industries that use chemical additives as preservatives or as food components. Mercury and lead have been identified many times as the dangerous elements whose exposure at pregnancy predisposes to teratogenicity. I consider the article findings on chemicals in food, fuel or any other source substantially convincing and policy makers should work around the clock to prevent exposure as an important step in prevention against autism and related conditions. The article explores nutrition, autism and how a combination of nutritional supplements can benefit autistic children. It further reports experimental studies that help affirm suggestion that vitamin B6 and Magnesium element combination is more

Biochemistry Lab Report Example | Topics and Well Written Essays - 750 words

Biochemistry - Lab Report Example The gels were visualized by coomassie staining. The restriction digestion of recombinant DNA yielded the predicted band sizes upon electrophoresis, confirming the presence of the pGLO plasmid in the transformants. The presence of a single neat band at the expected size range upon immunoblotting indicates the successful isolation of the purified GFP protein. Initially obtained from the jellyfish Aequorea victoria/Aequorea aequorea/Aequorea forskalea, the Green fluorescent protein (GFP) is composed of 238 amino acids with a molar mass of 26.9 kDa. Its typical three dimensional structure facilitates a specific set of cyclization reactions inside the protein giving rise to the tripeptide Ser65-Tyr66-Gly67 which forms the fluorophore, the fluorescent component of the protein, present on the alpha helix. This helix is actively shielded from the surrounding environment by the beta sheets, hence contributing to the use of the GFP gene as a reporter of gene expression or cellular protein localization. In the present experiment, the GFP gene has been used to understand the mechanism of molecular cloning and subsequent protein purification procedures. The molecular cloning was carried out by transforming the pGLO plasmid vector with the GFP gene insert into the bacterial host E.coli strain HB101. ... After appropriate incubation, the colonies were observed under normal light, followed by U.V light. Under normal light all the plates except plate with LB+Amp had growth Lack of plasmid DNA on Plate with LB+Amp-pGLO might have rendered the colonies susceptible to the antibiotic ampicillin, due to absence of genes required to deactivate ampicillin. Flourescence was observed in plates 3 and 4 under U.V light indicating the transformation of the plasmid DNA into the E.coli host. Plate 3, inspite of having ampicillin showed growth due to the presence of B-lactamase gene which can inactivate ampicillin. Plate 4 had the maximum number of colonies compared to the other plates. This could be attributed to the addition of arabinose into the medium which selectively enhances the activity of the arabinose operon in which the GFP gene has been inserted, thus increasing the GFP protein which has the unique quality of fluorescence. To confirm the insertion of the pGLO plasmid into the colonies on plates 3 and 4, the DNA from these colonies was purified using QAI kit method and subjected to restriction digestion using EcoRV and Hind III followed by electrophoresis, wherein an electric field was applied to the gel matrix. DNA molecules move towards the anode due to negativity of the charged phosphates along its backbone. The rate of migration of a particular DNA fragment is inversely proportional to its molecular weight; hence the fragments with the highest weight have the least mobility. Post electrophoresis, the ethidium bromide stained gel was visualized under UV light (Figure 1). A single restriction site specific to EcoRV is present on